FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5544894
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG28150.060.6203163
AGTAGCT108500.060.1178785
CCGAGTA30600.057.6496662
GAGTAGC71450.056.8362124
GTAGCTG114900.056.142686
TTGGGAG167650.052.1768536
GCTGGGA231400.051.1186529
CCCGAGT37900.050.078381
TAGCTGG136600.048.910547
GGAGGCT176200.046.6622169
GGGAGGC233100.043.6729288
TACTCGG31150.043.455833
CTACTCG31450.043.0416952
TTTGGGA163250.042.9106255
GCACTTT147050.041.6274571
TGGGAGG228000.041.018477
TCGGGAG38300.040.603196
CAAGTAG53850.040.2465823
AAGTAGC59300.039.7046434
CGGAGGT37250.039.6216667
Produced by FastQC (version 0.11.5)