FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5544894
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG28750.069.073873
CCGAGTA28950.067.353982
AGTAGCT114450.063.244795
GAGTAGC72500.061.432084
GTAGCTG120750.058.097856
CCCGAGT36450.055.0743331
TTGGGAG163350.054.3108376
GCTGGGA235750.054.122029
TAGCTGG139500.052.3611077
CAAGTAG54450.046.1179663
GCACTTT146450.045.6915781
CTACTCG31500.045.6837772
TTTGGGA160900.045.6575165
TACTCGG33200.044.211443
GGAGGCT185100.043.971699
GGGAGGC239000.043.5398948
ACTTTGG151400.043.1046373
AAGTGCT139550.042.485425
CCAAGTA61100.042.3939482
CACTTTG162800.041.4563452
Produced by FastQC (version 0.11.5)