FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5905286
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG26500.060.5510673
CCGAGTA27400.059.352
AGTAGCT100200.055.0077485
GAGTAGC63800.052.5566944
GTAGCTG107200.050.610356
CCCGAGT36450.048.167611
GCTGGGA212500.047.9198539
TTGGGAG155450.046.7512746
TAGCTGG122150.045.5411457
GGAGGCT171500.041.2903829
CGGAGGT35200.039.4577757
TACTCGG28900.039.3388863
GCACTTT145750.038.7546581
GGGAGGC218000.038.5901078
CTACTCG29150.037.2735862
TTTGGGA166300.036.9511875
GCTACTC76150.036.379241
GGAGGTT89850.036.2024778
CACTTTG155150.035.8037342
TGGGAGG219450.035.5477147
Produced by FastQC (version 0.11.5)