FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4843502
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG31000.072.4685063
AGTAGCT114800.071.9330755
GAGTAGC73050.070.67334
CCGAGTA32400.070.2268142
GTAGCTG119550.068.171676
CCCGAGT41250.058.852671
GCTGGGA236800.058.7094049
TTGGGAG159300.058.6632356
TAGCTGG145150.057.636317
CCAAGTA56700.051.4319342
TTTGGGA157200.050.8367235
CAAGTAG54900.049.7076533
GGAGGCT181350.049.6645859
GCACTTT136650.049.6376841
TGAGTAG58850.047.9618573
CTGAGTA61400.047.7295072
CTACTCG32900.047.273612
AAGTAGC58100.047.2177474
ACTTTGG146300.046.509743
TACTCGG34250.046.0404933
Produced by FastQC (version 0.11.5)