FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4843502
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG30350.080.605953
AGTAGCT110500.075.794485
CCGAGTA33600.074.308262
GAGTAGC71800.072.3535164
GTAGCTG116650.070.1330266
GCTGGGA239450.061.9415059
TTGGGAG174700.061.7384346
CCCGAGT42200.059.8468861
TAGCTGG145150.057.85927
TTTGGGA164350.055.688345
GCACTTT144650.055.3137661
CAAGTAG49700.055.1588823
ACTTTGG149550.053.4051863
AAGTGCT133500.053.196475
CCAAGTA54250.053.1852872
CACTTTG154200.051.9347082
AAGTAGC56300.050.226284
GGAGGCT195150.050.2258349
GGGAGGC263250.049.3432128
CTACTCG35100.047.968442
Produced by FastQC (version 0.11.5)