FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5783232
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA28400.068.200662
CGAGTAG28050.068.024273
AGTAGCT109800.064.914055
GAGTAGC74650.059.1264154
GTAGCTG120200.058.6985636
TTGGGAG168600.054.1034366
TAGCTGG138000.052.744597
GCTGGGA234500.052.622789
CCCGAGT40850.050.1942181
GGAGGCT178350.045.373169
TTTGGGA172500.043.781665
GGGAGGC239100.043.5707248
CGGAGGT37950.042.8748367
GGAGGTT91700.042.7890858
GCACTTT155250.042.422041
AAGTAGC61400.042.0995064
TGGGAGG239450.041.342217
CAAGTAG59300.041.0405243
CCAAGTA61650.040.644412
ACTTTGG160150.040.463783
Produced by FastQC (version 0.11.5)