FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4537804
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT70800.045.4548455
CGAGTAG19200.045.375723
CCGAGTA20000.044.640712
GAGTAGC46850.042.8779534
GTAGCTG75450.042.2717676
CGGAGGT20000.040.677517
GCTGGGA140600.039.991869
TAGCTGG85400.039.5385637
TTGGGAG99700.038.633546
CCCGAGT25700.037.777911
GGAGGTT54550.034.315048
GGAGGCT109300.033.593559
CTACTCG17200.033.4889032
TACTCGG19550.033.146313
GGGAGGC140750.032.890358
TGGGAGG137950.030.2699837
TCGGGAG25550.030.1507976
GCTACTC46600.030.0080361
GCACTTT97500.029.2791061
GAGGTTG75500.029.1796729
Produced by FastQC (version 0.11.5)