FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6417618
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG26650.051.5700263
CCGAGTA27700.051.1737982
AGTAGCT113900.050.4759255
GAGTAGC71150.050.1612054
GCTGGGA224400.047.723919
GTAGCTG119500.046.966476
TTGGGAG164550.045.6086926
TAGCTGG133100.044.393677
TACTCGG28950.040.7620963
CCCGAGT38100.040.415691
CGGAGGT35950.040.239557
GGAGGCT174050.039.6138849
GGGAGGC221850.038.507678
CTACTCG30450.037.8088842
GGAGGTT91600.036.2996528
TGGGAGG223400.036.2108847
TTTGGGA168300.035.7851265
AAGTGCT144900.035.605085
GCTACTC76900.035.5564541
GCACTTT148850.035.530371
Produced by FastQC (version 0.11.5)