FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4365536
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA16050.050.660222
CGAGTAG16250.048.2655033
AGTAGCT66450.042.7726145
GCTGGGA128750.042.539339
GAGTAGC41550.041.562714
CCCGAGT22800.040.0804441
TTGGGAG97800.039.877156
CGGAGGT22700.039.6312147
GTAGCTG70150.039.5934456
TAGCTGG76800.038.890197
GGAGGCT109650.036.4938249
GGAGGTT55400.035.8552328
GGGAGGC128750.035.8314178
TACTCGG18450.035.490223
CTACTCG17600.034.342372
TGGGAGG129300.032.7289437
GCTACTC44050.032.5065421
AGGCGGA31200.031.826454
GCACTTT92200.030.4366861
GGCGGAG33650.029.9368865
Produced by FastQC (version 0.11.5)