FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4166322
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG16100.046.0632673
CCGAGTA17350.044.820642
AGTAGCT61400.044.557635
TTGGGAG96950.041.585996
GTAGCTG66700.041.1250086
GAGTAGC40150.040.170374
GCTGGGA125850.039.5876549
TAGCTGG77550.037.7850047
CCCGAGT24050.036.4335821
GGAGGCT104500.036.37689
GGGAGGC128400.035.4932338
CTACTCG17950.035.299782
GGAGGTT51800.034.7469868
CGGAGGT21300.034.476847
TACTCGG18050.034.305573
TGGGAGG129150.033.336017
CTCGGGA19550.032.7755625
GCTACTC44800.032.4899941
TTTGGGA105100.032.1960075
TCGCGTT1352.2486005E-531.998115
Produced by FastQC (version 0.11.5)