FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4822174
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT105800.067.912445
GTAGCTG107550.065.6024556
GAGTAGC65650.064.821684
CCGAGTA26200.064.586022
CGAGTAG25950.062.4327773
TAGCTGG128450.056.0493167
TTGGGAG146850.054.3213166
GCTGGGA212800.053.9965139
CAAGTAG49150.048.492213
CCCGAGT36850.047.5705571
AAGTAGC55450.045.969444
TACTCGG29950.045.9202273
GGAGGCT164650.045.9126179
CTACTCG29050.045.8561442
CCAAGTA53750.045.6822972
TTTGGGA148700.045.4146845
TGAGTAG55950.045.301243
GCACTTT132000.044.6694761
CGGAGGT34900.043.9398547
CTGAGTA57700.043.5533562
Produced by FastQC (version 0.11.5)