FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4822174
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT102750.069.046975
CCGAGTA27300.068.790332
GAGTAGC65450.066.951024
CGAGTAG27050.066.7661063
GTAGCTG106050.065.744996
TTGGGAG154600.058.5026256
GCTGGGA214150.058.4756859
CCCGAGT35000.057.1513251
TAGCTGG125900.056.1345067
TTTGGGA149500.049.8136865
CAAGTAG50600.049.2011343
GCACTTT130200.049.0740131
CCAAGTA53550.049.0436672
AAGTGCT124650.048.026465
ACTTTGG135350.046.9409643
GGGAGGC224650.046.6443568
TGAGTAG54300.046.5111243
AAGTAGC55300.046.06044
TGGGAGG210350.045.8465237
GGAGGCT168100.045.5959249
Produced by FastQC (version 0.11.5)