FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4684245
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT83650.051.8993875
GAGTAGC54250.049.6370434
CCGAGTA20800.049.1540222
GTAGCTG91500.048.6270876
GCTGGGA169150.046.394389
CGAGTAG21000.045.943023
TAGCTGG102800.044.9627387
TACTCGG22300.044.5562133
CTACTCG23050.044.3559072
CCCGAGT27450.041.4343531
TTGGGAG123400.041.3075076
GGAGGCT132400.039.913429
GCTACTC57150.038.788991
CTCGGGA26550.037.1506925
TCGGGAG26700.036.941986
CGGAGGT28000.035.9982077
GGAGGTT69950.035.0976078
GGGAGGC166800.035.0486158
GCACTTT108700.034.1227261
TGGGAGG167200.033.2854237
Produced by FastQC (version 0.11.5)