FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4684245
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT80500.052.112755
CCGAGTA21050.050.9337352
CGAGTAG20700.050.404463
GAGTAGC51350.049.605994
GTAGCTG86550.049.1350866
GCTGGGA166500.047.0288969
TAGCTGG100700.044.3110857
TTGGGAG123100.044.0742236
CTACTCG23150.042.272632
CCCGAGT28600.042.0166851
TACTCGG23150.040.407663
GGAGGCT139550.040.3298959
GCTACTC57950.038.492811
GGGAGGC177950.036.520778
GCACTTT109900.036.2729951
TTTGGGA125550.035.7061235
CTCGGGA28800.035.4786575
TCGGGAG30950.035.3396
TGGGAGG170600.034.8036737
GGAGGTT68250.034.271328
Produced by FastQC (version 0.11.5)