FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4396364
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG17050.048.6001633
CCGAGTA17450.048.3119662
AGTAGCT65250.043.4739575
GTAGCTG66900.041.8636366
GCTGGGA123200.040.6151059
GAGTAGC40800.040.4182664
TTGGGAG100100.039.4148566
CTACTCG18100.039.4113122
CCCGAGT24400.038.208611
GGAGGCT103050.037.3782739
TACTCGG19600.037.1303983
TAGCTGG76700.036.8901677
CTCGGGA20300.036.530425
CGGAGGT21300.035.8294147
GCTACTC47900.035.6072041
GGGAGGC132100.035.371658
TCGGGAG22150.035.1045576
GGAGGTT54850.033.9966858
GCACTTT97700.032.0298351
TGGGAGG132250.031.7929367
Produced by FastQC (version 0.11.5)