FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4305835
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT113750.079.8750155
CGAGTAG31450.077.152083
GAGTAGC73250.075.195554
GTAGCTG121400.074.72316
CCGAGTA33450.072.755192
GCTGGGA243700.066.352499
TAGCTGG145000.064.994217
TTGGGAG171200.064.761966
CCAAGTA52200.061.5187842
CCCGAGT40550.060.2031
CAAGTAG51750.058.8529663
TTTGGGA158450.058.0686385
GCACTTT141400.057.941841
AAGTAGC55400.056.0151944
ACTTTGG150050.055.3263053
GGAGGCT185250.054.0206269
AAGTGCT128100.053.3921625
CACTTTG153250.053.3259742
CTGAGTA58100.053.040922
TGAGTAG57100.051.447283
Produced by FastQC (version 0.11.5)