FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4305835
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA31150.081.999912
CGAGTAG30800.081.296463
AGTAGCT116600.081.20835
GAGTAGC75600.077.948044
GTAGCTG123350.076.3560646
TTGGGAG169300.067.872066
GCTGGGA257700.066.2172559
TAGCTGG149800.065.532487
GCACTTT137300.065.29661
CCCGAGT41150.062.4225431
TTTGGGA157450.061.372875
CACTTTG143700.061.286912
CAAGTAG50000.060.871423
ACTTTGG147400.060.041393
TGAGTAG58200.056.7456743
AAGTGCT133700.056.668285
CCAAGTA53850.056.1185762
GGAGGCT183200.055.8635039
CTGAGTA60150.055.6237682
CTTTGGG167300.054.7059144
Produced by FastQC (version 0.11.5)