FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4450292
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG29750.079.381343
CCGAGTA31350.074.890012
AGTAGCT103450.071.4082645
GAGTAGC67450.070.665534
GTAGCTG108800.067.235156
CCCGAGT36550.066.082981
GCTGGGA221400.058.536469
TTGGGAG154700.058.1769946
TAGCTGG130400.056.6501857
GGAGGCT164650.050.7694059
CAAGTAG48800.050.458913
GCACTTT129750.050.1223451
CCAAGTA50100.049.881052
TTTGGGA147100.048.9461945
ACTTTGG132900.048.4333083
CACTTTG137250.047.2776952
GGGAGGC225650.046.712958
GCTACTC71550.045.6478961
AAGTAGC53850.045.325814
AAGTGCT124950.045.1763765
Produced by FastQC (version 0.11.5)