FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4274927
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG21750.071.218143
CCGAGTA24750.065.20552
AGTAGCT86050.064.508995
GAGTAGC54400.062.7440224
GTAGCTG93800.058.9488146
TAGCTGG107600.053.395837
GCTGGGA180950.051.9638529
CCCGAGT32300.050.7644421
TTGGGAG123600.049.6875156
TACTCGG25950.046.0873953
CTACTCG25150.044.6887362
CAAGTAG43650.044.399643
GGAGGCT135200.042.974779
CCAAGTA47400.042.2549822
GCACTTT111650.041.923021
AAGTAGC46600.041.8770684
GGGAGGC175950.040.5100368
CTCGGGA28350.040.3794865
TTTGGGA124750.040.2839055
AGCTGGG149500.039.2494138
Produced by FastQC (version 0.11.5)