FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4274927
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG20900.066.141143
GAGTAGC56100.062.372074
AGTAGCT86250.062.0221065
CCGAGTA22850.060.1935922
GTAGCTG92850.057.9235886
GCTGGGA185650.054.3713159
TAGCTGG104700.053.9808547
TTGGGAG131450.052.690376
CCCGAGT29550.050.2586751
CTACTCG27900.047.4915922
GGAGGCT143950.047.464769
TACTCGG28600.046.823483
CTCGGGA28950.046.506095
GCACTTT117500.045.342751
GGGAGGC185750.044.7682278
TTTGGGA130400.044.4462435
GCTACTC63100.044.216351
TCGGGAG32500.043.4199646
AAGTGCT111850.042.612675
GGAGGTT74250.041.7924238
Produced by FastQC (version 0.11.5)