FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4843825
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1251.8189894E-1257.5969285
CGAGTAG17400.045.136453
CTCGCGT2100.044.5764124
CCGAGTA18650.043.270252
TTGGGAG107700.039.8418856
CGGAGGT19050.038.927067
TACTCGG19650.038.1347543
AGTAGCT72900.037.9239045
CTACTCG18950.037.64232
GGGAGGC137050.036.9830678
GAGTAGC45000.036.6440964
GTAGCTG73500.036.4388736
GCTGGGA129350.036.0676549
CCCGAGT26250.035.2347261
GGAGGCT113700.035.0799269
CTCGGGA21400.034.9887855
TAGCTGG80300.034.249737
TCGGGAG24850.033.3181426
GGAGGTT56450.033.0327848
TGGGAGG141700.031.349077
Produced by FastQC (version 0.11.5)