FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4534980
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG18850.060.010733
CCGAGTA20700.058.8243332
AGTAGCT82050.056.1579865
GAGTAGC53050.054.8399544
GTAGCTG90550.051.522476
GCTGGGA168350.050.3353969
TTGGGAG127950.049.573066
TAGCTGG102400.046.966337
CCCGAGT27850.046.718561
CGGAGGT27100.044.366727
TACTCGG24000.044.13133
GGAGGCT128400.043.904289
GGGAGGC172350.042.107498
CTACTCG24450.041.5509452
TCGGGAG27050.041.5209666
GCACTTT116700.041.4378661
CTCGGGA26350.040.4381455
GGAGGTT70450.040.2647828
TTTGGGA130250.039.853615
TGGGAGG167850.038.9470567
Produced by FastQC (version 0.11.5)