FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6025211
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG25800.028.184973
CTACTCG26700.028.0524862
CGAGTAG23600.027.7616523
AGTAGCT100450.027.4514165
GAGTAGC62000.026.7086584
CCGAGTA25350.026.4213072
GTAGCTG105100.025.6888396
GCTGGGA197150.025.380729
TTGGGAG141000.025.0203236
TAGCTGG120100.024.2188957
CTCGGGA33550.021.459695
TCGCGTT3055.719612E-721.2450925
GGAGGCT168100.021.0723679
CCCGAGT38200.020.9477861
GGGAGGC198750.020.6844948
TGGGAGG202700.019.286887
TCGGGAG38250.019.1992686
CAAGTAG52750.018.9717883
ACTCGGG40750.018.7281194
CCCAAAG152900.018.718091
Produced by FastQC (version 0.11.5)