FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4763167
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA19900.051.3915522
CGAGTAG19850.050.7793543
GAGTAGC48650.047.5052764
AGTAGCT79450.046.8507235
CGGAGGT25950.045.5015457
TTGGGAG115950.045.4527326
GTAGCTG84050.043.5156676
GCTGGGA154300.043.3013739
CTACTCG18300.042.503932
CCCGAGT27900.042.379161
TACTCGG19300.041.7811473
GGAGGCT121850.041.1247569
TAGCTGG95100.039.367927
GGGAGGC153600.038.4832348
AGGCGGA37850.036.5220384
TGGGAGG153250.036.2690737
GGAGGTT68300.035.7353978
GGCGGAG38400.035.6239435
GCACTTT107400.034.839811
TTTGGGA122650.034.810225
Produced by FastQC (version 0.11.5)