FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4618058
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG25850.068.570463
CCGAGTA27400.066.532292
GAGTAGC62950.065.888114
AGTAGCT102100.064.310045
GTAGCTG107450.059.49996
CCCGAGT35400.053.8986781
GCTGGGA208750.053.3548059
TAGCTGG124500.052.9128887
TTGGGAG148050.050.672116
GGAGGCT158650.042.8392269
TGAGTAG52650.042.8359573
TTTGGGA148200.042.7022135
GCACTTT130950.042.6630061
AAGTAGC51900.041.7618184
CAAGTAG48200.041.7080273
GGGAGGC213050.041.497948
CTACTCG29150.040.78572
CTGAGTA56150.040.679162
CCAAGTA52150.040.483292
ACTTTGG136900.039.9485363
Produced by FastQC (version 0.11.5)