FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4618058
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG26750.073.206763
AGTAGCT104200.070.544655
CCGAGTA28150.068.5629352
GAGTAGC67450.068.5265964
GTAGCTG111550.066.6709756
TAGCTGG130650.057.4201557
GCTGGGA223350.057.409539
CCCGAGT35050.056.7744521
TTGGGAG149250.055.377536
GGAGGCT169000.047.0314569
GCACTTT125950.046.9404831
CGGAGGT35750.046.923017
CAAGTAG50200.046.8975453
TGAGTAG56700.046.6003343
CTGAGTA56050.046.3836142
TTTGGGA143450.046.0731775
CCAAGTA52050.045.6589852
AAGTGCT128100.045.5241625
AAGTAGC55300.044.78574
CTACTCG30600.044.2455372
Produced by FastQC (version 0.11.5)