FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3456150
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG24150.079.9450763
CCGAGTA25250.078.7447742
AGTAGCT93450.075.965085
GAGTAGC58350.074.9099664
GTAGCTG97350.072.108256
CCCGAGT32550.065.002371
GCTGGGA192550.062.742939
TAGCTGG117750.061.93917
TTGGGAG134600.061.7272766
CAAGTAG40550.058.0939563
AAGTAGC44250.054.6786464
GCACTTT110500.054.3717881
TTTGGGA127500.053.8144765
CCAAGTA45550.053.7730332
GGAGGCT144950.052.998379
TGAGTAG44300.052.36323
AAGTGCT100450.051.1041225
ACTTTGG119150.050.4854773
CACTTTG119000.049.88322
CTCGGGA29300.049.636385
Produced by FastQC (version 0.11.5)