FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4994975
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG26900.069.5868453
AGTAGCT112150.068.304365
CCGAGTA29200.067.326472
GAGTAGC69050.066.000334
GTAGCTG117100.064.5562746
GCTGGGA234400.058.081799
TAGCTGG136850.057.3965847
CCCGAGT36650.056.8533971
TTGGGAG164800.055.2636266
CAAGTAG54700.048.6990473
TACTCGG33450.047.997083
GGAGGCT176100.047.7926679
GCACTTT144550.046.985661
CCAAGTA56900.046.700352
CTACTCG33150.046.270282
TTTGGGA160400.046.141835
CGGAGGT36000.045.5972257
GGGAGGC238300.045.3786938
ACTTTGG148350.044.939633
AAGTGCT135650.044.8479925
Produced by FastQC (version 0.11.5)