FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4560865
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1755.820766E-1141.1411135
CTCGCGT2150.040.192724
CCGAGTA17600.038.8888472
CGAGTAG16100.038.0370833
TACTCGG17650.035.513113
AGTAGCT63700.035.489865
TTGGGAG94550.034.875316
GCTGGGA117300.034.49479
GAGTAGC42100.033.3547064
GTAGCTG66400.033.070896
CTACTCG17550.032.4312822
GGAGGCT97900.031.8433939
CCCGAGT25950.031.7442591
CGGAGGT16800.031.284397
GAGGTTG63650.030.993199
GGGAGGC120000.030.5987058
GGAGGTT51050.030.0398658
CGCGTTG2651.5097612E-1029.8855276
TGGGAGG118700.029.5387677
GCTACTC42650.029.3106041
Produced by FastQC (version 0.11.5)