FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4560865
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG16350.044.035363
CCGAGTA17050.043.5042952
GAGTAGC41300.042.884894
AGTAGCT64200.042.2790875
TCGCGTT2600.041.53725
CGGAGGT19400.040.82357
TTGGGAG97950.040.7950866
GTAGCTG66800.040.525726
GCTGGGA120500.040.1514789
CTACTCG17850.039.133872
CTCGGGA19500.038.0296135
TACTCGG18400.037.9553683
GGAGGCT101750.037.2902689
GCTACTC44900.036.2939451
GGGAGGC125650.035.755388
GGAGGTT54900.035.2776158
TCGGGAG22300.035.1917576
TAGCTGG80500.034.254867
CCCGAGT25700.033.948791
AGGCGGA27450.033.3104634
Produced by FastQC (version 0.11.5)