FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4976261
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1851.8189894E-1242.8091625
CCGAGTA16900.037.943912
CGAGTAG16250.037.244723
CTCGCGT2351.8189894E-1236.77234
GCTGGGA132700.036.514049
TTGGGAG99500.034.5152556
AGTAGCT72150.034.227375
CCCGAGT26250.033.5759351
CTACTCG16650.032.888052
CGGAGGT17350.032.367637
GTAGCTG75500.032.136516
GAGTAGC44500.031.717834
GGGAGGC125150.031.3531678
GGAGGCT105800.031.2350929
TAGCTGG82550.030.5257787
TACTCGG17750.030.4443
CTCGGGA20300.029.7919585
GCTACTC46500.028.897321
TGGGAGG126950.028.8102347
GGAGGTT50150.028.569198
Produced by FastQC (version 0.11.5)