FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4976261
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG16000.050.39763
CCGAGTA17450.047.8722042
CGGAGGT22750.042.7232367
TACTCGG17050.040.5376553
AGTAGCT67500.039.9980935
GAGTAGC44750.039.2562264
CTACTCG16800.039.007942
TCGCGTT2400.038.9981425
GCTGGGA135450.038.1111379
CCCGAGT24950.037.854291
GTAGCTG73200.036.3917056
GGAGGTT57100.035.8091478
TTGGGAG105050.035.50146
TAGCTGG84100.033.815277
GGCGGAG33450.033.3616375
AGGCGGA32400.032.8873184
GGGAGGC138200.032.1952828
GCGGAGG30550.031.5795086
TGGGAGG134350.030.7599747
GGAGGCT110000.030.2385569
Produced by FastQC (version 0.11.5)