FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4650612
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG17400.043.4777643
CCGAGTA18950.042.9631772
GAGTAGC42550.042.1403434
AGTAGCT69100.042.093745
GCTGGGA135500.040.2757959
GTAGCTG72450.040.1473776
TTGGGAG103700.037.2829066
CGGAGGT20050.036.626897
CCCGAGT26550.036.4680141
TAGCTGG82750.036.368267
GGAGGTT54600.036.1303448
GGAGGCT108300.035.3004539
GGGAGGC129100.034.6879278
TACTCGG18700.034.6759263
CTACTCG19100.033.9497262
GAGGTTG72150.033.628529
TCGGGAG22000.032.3986366
GCTACTC47100.031.909081
CTCGGGA20950.031.2731465
TGGGAGG132200.030.7702627
Produced by FastQC (version 0.11.5)