FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4650612
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA18550.057.459972
CGAGTAG18700.056.982513
AGTAGCT70050.047.7932175
GTAGCTG71900.046.1629456
CCCGAGT26050.045.3929521
GAGTAGC44550.044.6049274
GCTGGGA136000.043.622429
TTGGGAG113200.043.376996
TAGCTGG86000.040.017597
CTACTCG19250.038.160762
GGGAGGC141000.037.88848
CGGAGGT21800.037.650427
GGAGGTT61200.036.8225988
GGAGGCT115250.036.420779
TACTCGG20800.035.652943
TGGGAGG146400.035.6056567
GCTACTC45800.034.94941
CTCGGGA20600.034.6010635
GCACTTT105900.033.906711
AGGCGGA32850.033.5333944
Produced by FastQC (version 0.11.5)