FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4089859
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG25800.082.948563
CCGAGTA27400.079.4197542
AGTAGCT105150.078.6038065
GAGTAGC64300.077.946064
GTAGCTG108650.074.216296
TAGCTGG129500.063.601487
GCTGGGA221350.062.1898239
CCCGAGT35200.060.151671
CCAAGTA52000.058.060782
CAAGTAG49950.057.7027663
AAGTAGC52450.057.251454
TTGGGAG148900.056.0885546
TGAGTAG53200.051.197923
GGAGGCT157750.049.929699
CTGAGTA54900.049.6125562
CCCAAGT65000.049.473131
CTACTCG29500.048.8517532
TCGGGAG34300.048.6972966
TTTGGGA143600.047.9809045
GCACTTT123450.047.8248521
Produced by FastQC (version 0.11.5)