FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4089859
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG27550.085.45473
AGTAGCT103800.081.221185
GAGTAGC66800.080.941874
CCGAGTA29950.080.551682
GTAGCTG109900.075.730346
TAGCTGG131150.063.8441667
GCTGGGA227400.062.8779759
CCCGAGT39050.062.2130971
TTGGGAG157050.060.5584376
CAAGTAG45650.060.2465743
CCAAGTA49500.057.612482
AAGTAGC51200.054.98154
TGAGTAG51600.054.13673
TTTGGGA143600.053.0947155
GCACTTT125000.052.884971
GGAGGCT171750.052.60869
ACTTTGG131050.051.641713
CTGAGTA53900.051.3061072
AAGTGCT124900.050.1494985
CACTTTG133500.049.8984532
Produced by FastQC (version 0.11.5)