FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4906818
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA19100.036.9455682
CGAGTAG18850.036.2851373
AGTAGCT71550.035.6213345
GCTGGGA130300.035.5290079
CTACTCG17950.034.498772
GAGTAGC46750.034.4971284
GTAGCTG73700.033.9960336
TACTCGG19150.033.0849463
CGGAGGT21000.032.2273867
TAGCTGG81350.031.2416297
CCCGAGT27400.030.780721
TTGGGAG101050.030.4234246
AGGCGGA29000.030.0402744
ATTCGCG1204.2292368E-429.9988984
GGAGGCT109950.029.4013759
GGGAGGC131500.028.5799378
CTCGGGA21000.027.7704075
TCGGGAG24500.027.6234726
GGAGGTT54250.027.47188
GGCGGAG31400.027.2856225
Produced by FastQC (version 0.11.5)