FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4865383
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG16100.044.7488023
CCGAGTA17250.043.0185172
CGGAGGT17600.039.6800047
GAGTAGC41300.039.4033854
GCTGGGA132000.038.670969
AGTAGCT69400.037.9694445
GTAGCTG72350.036.9188356
CCCGAGT24400.035.8480641
TTGGGAG106750.035.5431026
GGAGGTT54950.034.19688
TAGCTGG82200.033.983857
GGGAGGC130250.032.9443668
TACTCGG17850.031.8857153
GGAGGCT104400.031.791659
GAGGTTG68700.031.3348129
TCGGGAG21300.031.0971686
TGGGAGG130050.030.2823527
CTACTCG17250.028.818232
GGCGGAG28300.028.238995
CTCGGGA20750.027.7577675
Produced by FastQC (version 0.11.5)