FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4865383
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG16350.044.0356863
CCGAGTA16500.043.647472
GAGTAGC42500.040.6578874
CGGAGGT20500.039.6868937
TTGGGAG106500.038.939956
AGTAGCT71300.038.4731675
TCGCGTT2300.037.5643545
GTAGCTG75150.037.3644146
CCCGAGT24350.037.316031
GCTGGGA135650.037.312829
GGAGGCT106000.035.3878679
GGGAGGC130800.035.1735048
GGCGGAG30650.035.000835
GGAGGTT60050.034.170748
TAGCTGG85100.034.0955737
AGGCGGA31450.032.9658554
TGGGAGG135750.032.14077
GCGGAGG26300.032.0296826
CTACTCG17850.031.873662
GAGGTTG78600.031.4191259
Produced by FastQC (version 0.11.5)