FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5753907
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT24650.043.8115657
CCGAGTA20500.039.7103082
CGAGTAG19700.039.4944653
AGGCGGA37700.037.817684
GGCGGAG37150.037.7911645
GCTGGGA156050.036.3560689
AGTAGCT81000.036.3540425
GAGTAGC50650.036.2519874
GGAGGTT67550.035.9185648
TACTCGG21950.034.4614453
GTAGCTG85300.034.0156
GCGGAGG32950.033.868096
TTGGGAG123900.033.528876
TCGCGTT2155.2568794E-1033.486985
TAGCTGG95850.032.374247
CTACTCG21600.032.351672
GAGGTTG90950.031.981089
GGAGGCT130150.031.863459
CTCGGGA23750.031.8302565
TCGGGAG26250.031.5415466
Produced by FastQC (version 0.11.5)