FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4988536
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT20250.044.7977687
CGAGTAG18300.043.308223
CCGAGTA19950.042.2543752
TAGCGCG1054.0150135E-641.140815
AGGCGGA30350.037.9617464
GAGTAGC45350.037.631884
AGTAGCT72100.037.1465535
GCTGGGA128700.036.585599
CCCGAGT27750.036.4500161
GGCGGAG32050.036.1666875
GCGGAGG27700.035.8682486
TTGGGAG105450.035.2301066
GTAGCTG76250.035.124816
TACTCGG19200.034.8987963
TCGCGTT2104.0745363E-1034.2840045
TAGCTGG83200.032.7964447
GGAGGTT58200.032.781878
CTACTCG19300.031.7314832
GGAGGCT108750.030.917089
GGGAGGC134650.030.691388
Produced by FastQC (version 0.11.5)