FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4988536
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA19450.043.3202062
CGAGTAG18950.042.173333
GAGTAGC45050.039.155744
GCTGGGA137950.038.465379
TTGGGAG103900.038.2514276
AGTAGCT73600.038.053615
GTAGCTG75100.036.6224636
CTACTCG19850.035.916912
GGAGGCT109250.034.9942939
TACTCGG20850.034.8770263
CCCGAGT27950.034.8179441
TAGCTGG82750.034.8029567
GGGAGGC133600.034.5442438
CGGAGGT22800.033.7888227
TCGCGTT2456.002665E-1132.3259055
TGGGAGG137050.032.3087547
CTCGGGA22100.031.9269855
AGGCGGA30900.031.9217154
GGAGGTT58750.031.3730138
GCTACTC49000.031.335361
Produced by FastQC (version 0.11.5)