FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4491218
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG16900.044.3369943
CCGAGTA19150.044.0186462
GAGTAGC43850.044.0101284
TTGGGAG113950.043.9754036
CTACTCG20150.043.6218832
AGTAGCT70950.043.1272165
TACTCGG22050.042.80383
GCTGGGA140900.041.389389
GTAGCTG73600.040.987476
TCGGGAG24250.039.7839556
CTCGGGA22650.039.733495
GGGAGGC144400.039.6382838
GGAGGCT117900.039.387729
GCTACTC51700.038.2994731
CCCGAGT25450.037.765621
TAGCTGG82800.037.7376027
CGGAGGT22750.037.0270737
GGAGGTT60600.036.7113848
TGGGAGG147200.035.7050747
TTTGGGA119600.033.95185
Produced by FastQC (version 0.11.5)