FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4491218
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGT2550.053.6450734
CGAGTAG17400.052.9635623
TCGCGTT2050.052.6809845
CCGAGTA19200.050.2626952
TTGGGAG116250.047.936296
GAGTAGC44450.047.134374
AGTAGCT71350.046.215535
GCTGGGA144050.044.5328949
GTAGCTG74950.043.995716
CCCGAGT25500.043.8230971
GGAGGCT116400.043.0499539
CGGAGGT28300.041.977257
GGGAGGC152000.041.2090038
TAGCTGG86900.039.0227247
AGGCGGA36000.037.5986144
GGAGGTT69500.037.2935878
GCTACTC49950.036.9501341
TTTGGGA124550.036.880215
GCACTTT105300.036.7669371
CTACTCG19400.036.380322
Produced by FastQC (version 0.11.5)