FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4906017
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1650.052.3611535
CCGAGTA18750.043.8087462
CGAGTAG17800.043.313283
GAGTAGC45450.040.0862924
CGGAGGT22750.039.2420967
AGTAGCT75150.038.992165
CTCGCGT3150.038.864014
GTAGCTG78200.038.392046
TTGGGAG108400.038.057236
GCTGGGA141850.037.914319
TAGCTGG86950.035.4393777
TACTCGG17800.034.8125423
GGAGGTT62100.034.7809568
CCCGAGT28300.034.478891
GGGAGGC135250.034.3880168
GGAGGCT114700.033.7072039
AGGCGGA32250.033.2709964
CTACTCG18150.032.156272
GGCGGAG35000.031.6784955
TGGGAGG137550.031.405277
Produced by FastQC (version 0.11.5)