FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4743385
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA18400.057.9193232
CGAGTAG17850.054.048873
CCCGAGT26150.046.309561
AGTAGCT71000.046.240925
GAGTAGC44600.046.1690754
CGGAGGT26000.042.921857
TTGGGAG115100.042.598266
GTAGCTG75900.042.4968036
GCTGGGA144850.042.249399
TAGCTGG86450.038.809767
AGGCGGA37050.038.476634
GGCGGAG40450.037.734395
GGAGGCT120150.037.272349
GGAGGTT64700.037.056128
GGGAGGC151650.036.889158
CTCGGGA24650.035.3417855
TGGGAGG151200.034.9989977
TACTCGG22600.034.7246673
TCGGGAG28250.033.6415146
CTACTCG22300.033.5821152
Produced by FastQC (version 0.11.5)