FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5430076
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT87750.030.0293795
CGAGTAG22900.029.2622643
GCTGGGA168450.027.6959579
GTAGCTG92750.027.63436
GAGTAGC55050.027.4694734
TAGCTGG104000.025.6834267
CCGAGTA26300.025.479312
CTACTCG22950.025.4309622
TACTCGG24350.024.2647233
TTGGGAG120550.023.590786
GCTACTC59250.022.0776751
CCCGAGT35800.022.004251
CTCGGGA28100.021.778355
GGAGGCT143200.021.3174489
CGGAGGT24950.021.0651577
TCGGGAG31100.020.6035486
GGGAGGC164150.020.3511738
CCCAAGT60550.020.1713831
CAAGTAG47250.020.129483
CCCAAAG134350.019.7958091
Produced by FastQC (version 0.11.5)