FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4426056
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG15350.048.8140953
CCGAGTA16300.046.4111142
GAGTAGC40900.044.722344
CGGAGGT21400.044.0730937
TCGCGTT1653.092282E-1143.634715
GTAGCTG67150.042.9946446
AGTAGCT67150.042.672995
GCTGGGA131300.040.6869549
TTGGGAG99150.039.8653536
TAGCTGG77600.039.1531527
TACTCGG17100.037.9198573
CCCGAGT23400.037.046391
GGAGGTT55700.036.58038
GGAGGCT102800.035.9285969
AGGCGGA29850.034.74024
GGCGGAG30750.034.652345
GGGAGGC131150.034.6399358
CTACTCG17300.034.5662542
CTCGGGA19000.034.48295
GAGGTTG68850.033.0444959
Produced by FastQC (version 0.11.5)