FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4668831
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA19900.047.792692
CGAGTAG19000.047.0228233
AGTAGCT76450.043.8852655
GAGTAGC49200.043.61644
CCCGAGT26550.040.5576061
GTAGCTG78750.040.4093636
GCTGGGA140600.039.48029
TTGGGAG108350.038.1411066
TAGCTGG85750.038.0342067
CGGAGGT20100.037.968217
CTACTCG19600.037.1283762
GGAGGCT111900.036.673739
TACTCGG20550.036.113213
CTCGGGA22800.035.9981585
GGGAGGC141450.034.254888
GGAGGTT57900.034.07088
GCTACTC45350.032.5089871
TGGGAGG139050.031.739487
TCGGGAG25100.031.5521726
AGGCGGA31150.029.5903854
Produced by FastQC (version 0.11.5)