FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4122201
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG30600.081.879433
AGTAGCT117600.081.058335
CCGAGTA32700.078.631312
GAGTAGC74950.077.80914
GTAGCTG124000.077.281116
GCTGGGA251350.068.402579
TAGCTGG147200.066.421547
TTGGGAG170100.066.241026
CAAGTAG48600.062.2199973
CCCGAGT42350.061.4466061
CCAAGTA50700.061.085142
GCACTTT136500.059.8330761
TTTGGGA153950.059.1599545
AAGTAGC53900.059.040564
ACTTTGG141250.057.5469743
AAGTGCT130600.057.4987145
GGAGGCT188250.057.0242589
CTGAGTA57400.056.4645232
TGAGTAG56800.056.4064373
CACTTTG147400.054.9216882
Produced by FastQC (version 0.11.5)